Identifying novel resistance genes from canola relatives and developing canola germplasm with multiple resistance genes sourced from B. nigra, B. juncea, and B. carinata

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Key Result

Over 10 lines that showed excellent resistance to aggressive isolates of the blackleg pathogen were produced.

Project Summary

Objectives:

To use cloned blackleg resistance genes to:

  1. identify the effective resistance genes.
  2. monitor the changing of pathogen isolates in canola fields.
  3. guide pyramiding effective resistance genes in the development of canola cultivars.
  4. guide the deployment of canola cultivars with various blackleg resistance genes.
  5. identify novel blackleg resistance genes in canola relative species.

Overview:

The resistance (R) gene sequences on chromosome N7 were sequenced and functionally confirmed. (They had been studied for three decades but not previously sequenced due to the structural complexity of this chromosome.) Over 10 advanced backcrossing lines (BC5 and BC6) were produced in a susceptible cultivar, Westar, background. All of these lines showed excellent resistance to aggressive isolates of the blackleg pathogen.

Note: This study was one of many making up the ‘Canola Disease Management Tools for the Prairies – Blackleg and Sclerotinia’ project that was led by SaskCanola in partnership with industry and funded under the Agri-Science Project (ASP) within the Growing Forward 2 Program.